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KMID : 0363020110410020054
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Journal of Korean Academy of Periodontology
2011 Volume.41 No. 2 p.54 ~ p.59
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Identification of mono- or poly-specific monoclonal antibody to Porphyromonas gingivalis heat-shock protein 60
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Choi Jeom-Il
Lee Sang-Yull
Kim Koan-Hoi
Choi Bong-Kyu
Kim Myung-Jin
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Abstract
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Purpose: The aim of this study was to define the immunoreactive specificity of Porphyromonas gingivalis (P. gingivalis) heat shock protein (HSP) 60 in periodontitis and atherosclerosis.
Methods: In an attempt to define the cross-reactive bacterial heat-shock protein with human self-antigen at molecular level, we have introduced a novel strategy for cloning hybridoma producing anti-P. gingivalis HSP 60 which is polyreactive to bacterial HSPs or to the human homolog.
Results: Five cross-reactive clones were obtained which recognized the #19 peptide (TLVVNRLRGSLKICAVKAPG) among 37 synthetic peptides (20-mer, 5 amino acids overlapping) spanning the whole molecule of P. gingivalis HSP 60. We have also established three anti-P. gingivalis HSP 60 monoclonal antibodies demonstrating mono-specificity. These clones recognized the #29 peptide (TVPGGGTTYIRAIAALEGLK).
Conclusions: Peptide #19 and #29 of P. gingivalis HSP 60 might be important immunoreactive epitopes in the immunopathogenic mechanism of bacterial antigen-triggered autoimmune diseases.
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KEYWORD
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Antibodies, Periodontitis, Porphyromonas gingivalis
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